Part:BBa_K2680122

3G pLsrA

pLsrA promoter flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

LsrA is one of four genes in the lsr quorum-sensing operon that aids in the uptake of Autoinducer-2 (AI-2) into the cell. Specifically, "lsrA encodes the ATPase that provides energy for AI-2 transport." Phosphorylated AI-2 (phospho-AI-2) induces the lsr operon by binding to LsrR, which represses the operon.¹

William and Mary iGEM encountered the promoter for lsrA through our collaboration with Virginia iGEM. For more information, visit [http://2018.igem.org/Team:Virginia/Parts Virginia iGEM's wiki].

References

¹Xavier, K. B., & Bassler, B. L. (2005). Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. Journal of Bacteriology, 187(1), 238–248. http://doi.org/10.1128/JB.187.1.238-248.2005

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 41
  Illegal BsaI.rc site found at 154